CRISPR-Cas-Enhanced Microbial Biofuel Production from algae

Date:12 April 2022

Application

• Enhanced biofuel production from microbes by optimizing metabolic pathways using CRISPR-Cas technology.
• Improved yields of biofuels like butanol, ethanol, and biodiesel through gene editing in microorganisms and algae.

Description

This project explores the use of the CRISPR-Cas system for improving microbial biofuel production. It focuses on editing the genomes of microorganisms like Clostridium, Escherichia coli, and algae to enhance biofuel yields such as butanol, ethanol, and lipids. The CRISPR-Cas technology enables precise gene editing to optimize metabolic pathways and reduce by-products. The approach offers a sustainable solution to increase biofuel production efficiency and support the transition to renewable energy.

Pathway Description:

The process used in this project involves applying the CRISPR-Cas system to edit the genomes of microorganisms like Clostridium and E. coli for improved biofuel production. This system is used to knock out or modify specific genes to redirect metabolic pathways, enhance substrate utilization, and increase the production of biofuels such as butanol, ethanol, and butyrate. By using CRISPR-Cas, researchers can optimize microbial strains for higher yield and efficiency in biofuel fermentation processes. The editing is achieved through multiplex genome editing, enabling precise modifications to multiple genes simultaneously.